Lung-On-A-Chip: Treating Pulmonary Edema
(Ivanhoe Newswire) – Imagine a world where a microchip can replace entire organs. That’s our world! And now, researchers have made even more progress in this field. Researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University have mimicked pulmonary edema, an abnormal buildup of fluid in the air sacs of the lungs which leads to shortness of breath, in a microchip lined by living human cells. They used this "lung-on-a-chip" to study drug toxicity and identify potential new therapies to prevent this life-threatening condition.
The study offers further proof-of-concept that human "organs-on-chips" hold tremendous potential to replace traditional approaches to drug discovery and development. "Major pharmaceutical companies spend a lot of time and a huge amount of money on cell cultures and animal testing to develop new drugs," Donald Ingber, M.D., Ph.D., founding director of the Wyss Institute and senior author of the study, was quoted as saying. "But these methods often fail to predict the effects of these agents when they reach humans."
The lung-on-a-chip device, which the team first described only two years ago, is a crystal clear, flexible polymer about the size of a memory stick that contains hollow channels fabricated using computer microchip manufacturing techniques. Two of the channels are separated by a thin, flexible, porous membrane that on one side is lined with human lung cells from the air sac and exposed to air; human capillary blood cells are placed on the other side with medium flowing over their surface. A vacuum applied to side channels deforms this tissue-tissue interface to re-create the way human lung tissues physically expand and retract when breathing.
Wyss Technology Development Fellow Dongeun Huh, Ph.D., studied a cancer chemotherapy drug called interleukin-2—or IL-2 for short—in the lung-on-a-chip. A major toxic side effect of IL-2 is pulmonary edema, which is a deadly condition in which the lungs fill with fluid and blood clots.
When IL-2 was injected into the blood channel of the lung-on-a-chip, fluid leaked across the membrane and two tissue layers, reducing the volume of air in the other channel and compromising oxygen transport—just as it does in lungs of human patients when it is administered at the equivalent doses and over the same time course. Blood plasma proteins also crossed into the air channel, leading to the formation of blood clots in the air space, as they do in humans treated with IL-2.
But one result came as a surprise. It turns out the physical act of breathing greatly enhances the effects of IL-2 in pulmonary edema --"something that clinicians and scientists never suspected before," Ingber was quoted as saying. When the team turned on the vacuum attached to the chip to simulate breathing, it increased fluid leakage more than three-fold when treated with the clinically relevant IL-2 dose, and the Wyss team confirmed that the same response occurs in an animal model of pulmonary edema. This result could suggest that doctors treating patients on a respirator with IL-2 should reduce the tidal volume of air being pushed into the lungs, for example, in order to minimize the negative side effects of this drug.
Most exciting for the future of drug testing was the Wyss team's finding that "this on-chip model of human pulmonary edema can be used to identify new potential therapeutic agents in vitro," Ingber was quoted as saying. "In just a little more than two years, we've gone from unveiling the initial design of the lung-on-a-chip to demonstrating its potential to model a complex human disease, which we believe provides a glimpse of what drug discovery and development might look like in the future.”
Source: Science Translation Medicine, November 2012